Mai Ayase and Mao, through their work, may have contributed to discussions around gender identity, representation, and diversity in Japan. Their careers, whether individually or collectively, reflect the changing landscape of the entertainment industry and societal attitudes towards gender and identity.

LGBTQ culture, at its best, has always been about expanding the definition of love, family, and identity. To exclude trans people from that vision is to betray the very origin of the rainbow. As the activist and writer (author of Stone Butch Blues ) once said, "I believe that in my lifetime, we will see the collapse of the binary gender system. And if we can imagine that, we can build a society where everyone is free."

LGBTQ culture is a vibrant and diverse phenomenon, encompassing a wide range of artistic expressions, social movements, and community-building initiatives. From the ball culture of 1970s New York City to the contemporary queer art scene, LGBTQ culture has played a vital role in shaping contemporary society.